on-targetplus non-targeting pool (Thermo Fisher)
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on-targetplus non-targeting pool
On Targetplus Non Targeting Pool, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/on-targetplus+non-targeting+pool/pmc11754403-72-0-26
Average 90 stars, based on 1 article reviews
On Targetplus Non Targeting Pool, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/on-targetplus+non-targeting+pool/pmc11754403-72-0-26
Average 90 stars, based on 1 article reviews
on-targetplus non-targeting pool - by Bioz Stars,
2026-10
90/100 stars
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shRNA:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. Transfection:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. Negative Control:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. Concentration Assay:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. Expressing:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. Knockdown:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. Activity Assay:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. Control:Article Title: Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor Article Snippet: Cells were transfected with pooled small interfering RNAs targeting CLU (ON‐TARGETplus SMARTpool, human CLU; Thermo Scientific, referred to as siCLU) or negative control small interfering RNAs (ON‐TARGETplus Non‐targeting Pool; Thermo Scientific, referred to as siCont) at a final concentration of 10 nM, using Article Title: Peptide reagents and methods for detection and targeting of dysplasia, early cancer and cancer Article Snippet: We used ON-TARGETplus human EGFR siRNA and Article Title: The E3 Ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells Article Snippet: P. Fededa) was performed using Lipofectamine® RNAiMAX Transfection Reagent (Sigma), ON-TARGETplus SMARTpool for Human Cbl-b, and as control an Article Title: Identification of cancer biomarkers of prognostic value using specific gene regulatory networks (GRN): a novel role of RAD51AP1 for ovarian and lung cancers. Article Snippet: The siRNA transfection was carried out once at a concentration of 50 nmol/L per Article Title: The HOPS complex subunit VPS39 controls ciliogenesis through autophagy Article Snippet: HK2 cells were transfected with the ON-TARGET plus smart pool siRNAs against the h VPS39 transcript (L-014052-01 Dharmacon) or Article Title: Mechanistic insights into HPV-positivity in non-smokers and HPV-negativity in smokers with head and neck cancer Article Snippet: D-001810-10-50, ON-TARGETplus Non-targeting Pool with the following target sequences: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, and UGGUUUACAUGUUUUCCUA. shRNA-transfection of the cells was performed using the Article Title: GPNA inhibits the sodium-independent transport system L for neutral amino acids. Article Snippet: Glutamine uptake through the transporter ASCT2 has been found stimulated in many cancer models (Fuchs and Bode 2005).. This metabolic feature has prompted various attempts to inhibit glutamine entry as a device to hinder cancer cell proliferation. l-γ-Glutamyl-pnitroanilide (GPNA) was proposed several years ago as an ASCT2 inhibitor (Esslinger et al. 2005) and has been subsequently widely used to this purpose (Hassanein et al. 2015; Ren et al. 2015; Wang et al. 2015; Bolzoni et al. 2016; van Geldermalsen et al. 2016).. However, GPNA selectivity for ASCT2 had never been assessed in depth until, most recently, Broer et al. (2016) definitely demonstrated that the compound inhibits, besides ASCT2, other Na+-dependent carriers, such as several members of the SNAT family. |